Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches.
Identifieur interne : 000113 ( Main/Exploration ); précédent : 000112; suivant : 000114Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches.
Auteurs : Raja Sayeh [France] ; Jean Louis Birrien ; Karine Alain ; Georges Barbier ; Mokhtar Hamdi ; Daniel PrieurSource :
- Extremophiles : life under extreme conditions [ 1433-4909 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (physiologie), Analyse cytogénétique (MeSH), Animaux (MeSH), Archéobactéries (composition chimique), Archéobactéries (isolement et purification), Archéobactéries (physiologie), Biote (MeSH), Gènes d'ARN ribosomique (MeSH), Hydrogensulfite reductase (physiologie), Interactions microbiennes (physiologie), Oxidoreductases (physiologie), Proteobacteria (composition chimique), Proteobacteria (isolement et purification), Proteobacteria (physiologie), Sources thermales (composition chimique), Sources thermales (microbiologie), Techniques de culture (MeSH), Tunisie (MeSH).
- MESH :
- composition chimique : Archéobactéries, Proteobacteria, Sources thermales.
- isolement et purification : Archéobactéries, Proteobacteria.
- microbiologie : Sources thermales.
- physiologie : ARN ribosomique 16S, Archéobactéries, Hydrogensulfite reductase, Interactions microbiennes, Oxidoreductases, Proteobacteria.
- Analyse cytogénétique, Animaux, Biote, Gènes d'ARN ribosomique, Techniques de culture, Tunisie.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Animals (MeSH), Archaea (chemistry), Archaea (isolation & purification), Archaea (physiology), Biota (MeSH), Culture Techniques (MeSH), Cytogenetic Analysis (MeSH), Genes, rRNA (MeSH), Hot Springs (chemistry), Hot Springs (microbiology), Hydrogensulfite Reductase (physiology), Microbial Interactions (physiology), Oxidoreductases (physiology), Proteobacteria (chemistry), Proteobacteria (isolation & purification), Proteobacteria (physiology), RNA, Ribosomal, 16S (physiology), Tunisia (MeSH).
- MESH :
- chemical , physiology : Hydrogensulfite Reductase, Oxidoreductases, RNA, Ribosomal, 16S.
- geographic : Tunisia.
- chemistry : Archaea, Hot Springs, Proteobacteria.
- isolation & purification : Archaea, Proteobacteria.
- microbiology : Hot Springs.
- physiology : Archaea, Microbial Interactions, Proteobacteria.
- Animals, Biota, Culture Techniques, Cytogenetic Analysis, Genes, rRNA.
Abstract
Prokaryotic diversities of 12 geothermal hot springs located in Northern, Central and Southern Tunisia were investigated by culture-based and molecular approaches. Enrichment cultures for both aerobic and anaerobic microorganisms were successfully obtained at temperatures ranging from 50 to 75°C. Fourteen strains including four novel species were cultivated and assigned to the phyla Firmicutes (9), Thermotogae (2), Betaproteobacteria (1), Synergistetes (1) and Bacteroidetes (1). Archaeal or universal oligonucleotide primer sets were used to generate 16S rRNA gene libraries. Representative groups included Proteobacteria, Firmicutes, Deinococcus-Thermus, Thermotogae, Synergistetes, Bacteroidetes, Aquificae, Chloroflexi, candidate division OP9 in addition to other yet unclassified strains. The archaeal library showed a low diversity of clone sequences belonging to the phyla Euryarchaeota and Crenarchaeota. Furthermore, we confirmed the occurrence of sulfate reducers and methanogens by amplification and sequencing of dissimilatory sulfite reductase (dsrAB) and methyl coenzyme M reductase α-subunit (mcrA) genes. Altogether, we discuss the diverse prokaryotic communities arising from the 12 geothermal hot springs studied and relate these findings to the physico-chemical features of the hot springs.
DOI: 10.1007/s00792-010-0327-2
PubMed: 20835839
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000704
- to stream PubMed, to step Curation: 000700
- to stream PubMed, to step Checkpoint: 000710
- to stream Main, to step Merge: 000113
- to stream Main, to step Curation: 000113
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches.</title><author><name sortKey="Sayeh, Raja" sort="Sayeh, Raja" uniqKey="Sayeh R" first="Raja" last="Sayeh">Raja Sayeh</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, UBO-CNRS-IFREMER, Place Nicolas Copernic, 29280, Plouzané, France. rajasayeh@yahoo.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, UBO-CNRS-IFREMER, Place Nicolas Copernic, 29280, Plouzané</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Plouzané</settlement></placeName></affiliation></author><author><name sortKey="Birrien, Jean Louis" sort="Birrien, Jean Louis" uniqKey="Birrien J" first="Jean Louis" last="Birrien">Jean Louis Birrien</name></author><author><name sortKey="Alain, Karine" sort="Alain, Karine" uniqKey="Alain K" first="Karine" last="Alain">Karine Alain</name></author><author><name sortKey="Barbier, Georges" sort="Barbier, Georges" uniqKey="Barbier G" first="Georges" last="Barbier">Georges Barbier</name></author><author><name sortKey="Hamdi, Mokhtar" sort="Hamdi, Mokhtar" uniqKey="Hamdi M" first="Mokhtar" last="Hamdi">Mokhtar Hamdi</name></author><author><name sortKey="Prieur, Daniel" sort="Prieur, Daniel" uniqKey="Prieur D" first="Daniel" last="Prieur">Daniel Prieur</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20835839</idno><idno type="pmid">20835839</idno><idno type="doi">10.1007/s00792-010-0327-2</idno><idno type="wicri:Area/PubMed/Corpus">000704</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000704</idno><idno type="wicri:Area/PubMed/Curation">000700</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000700</idno><idno type="wicri:Area/PubMed/Checkpoint">000710</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000710</idno><idno type="wicri:Area/Main/Merge">000113</idno><idno type="wicri:Area/Main/Curation">000113</idno><idno type="wicri:Area/Main/Exploration">000113</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches.</title><author><name sortKey="Sayeh, Raja" sort="Sayeh, Raja" uniqKey="Sayeh R" first="Raja" last="Sayeh">Raja Sayeh</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, UBO-CNRS-IFREMER, Place Nicolas Copernic, 29280, Plouzané, France. rajasayeh@yahoo.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, UBO-CNRS-IFREMER, Place Nicolas Copernic, 29280, Plouzané</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Plouzané</settlement></placeName></affiliation></author><author><name sortKey="Birrien, Jean Louis" sort="Birrien, Jean Louis" uniqKey="Birrien J" first="Jean Louis" last="Birrien">Jean Louis Birrien</name></author><author><name sortKey="Alain, Karine" sort="Alain, Karine" uniqKey="Alain K" first="Karine" last="Alain">Karine Alain</name></author><author><name sortKey="Barbier, Georges" sort="Barbier, Georges" uniqKey="Barbier G" first="Georges" last="Barbier">Georges Barbier</name></author><author><name sortKey="Hamdi, Mokhtar" sort="Hamdi, Mokhtar" uniqKey="Hamdi M" first="Mokhtar" last="Hamdi">Mokhtar Hamdi</name></author><author><name sortKey="Prieur, Daniel" sort="Prieur, Daniel" uniqKey="Prieur D" first="Daniel" last="Prieur">Daniel Prieur</name></author></analytic><series><title level="j">Extremophiles : life under extreme conditions</title><idno type="eISSN">1433-4909</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Archaea (chemistry)</term><term>Archaea (isolation & purification)</term><term>Archaea (physiology)</term><term>Biota (MeSH)</term><term>Culture Techniques (MeSH)</term><term>Cytogenetic Analysis (MeSH)</term><term>Genes, rRNA (MeSH)</term><term>Hot Springs (chemistry)</term><term>Hot Springs (microbiology)</term><term>Hydrogensulfite Reductase (physiology)</term><term>Microbial Interactions (physiology)</term><term>Oxidoreductases (physiology)</term><term>Proteobacteria (chemistry)</term><term>Proteobacteria (isolation & purification)</term><term>Proteobacteria (physiology)</term><term>RNA, Ribosomal, 16S (physiology)</term><term>Tunisia (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (physiologie)</term><term>Analyse cytogénétique (MeSH)</term><term>Animaux (MeSH)</term><term>Archéobactéries (composition chimique)</term><term>Archéobactéries (isolement et purification)</term><term>Archéobactéries (physiologie)</term><term>Biote (MeSH)</term><term>Gènes d'ARN ribosomique (MeSH)</term><term>Hydrogensulfite reductase (physiologie)</term><term>Interactions microbiennes (physiologie)</term><term>Oxidoreductases (physiologie)</term><term>Proteobacteria (composition chimique)</term><term>Proteobacteria (isolement et purification)</term><term>Proteobacteria (physiologie)</term><term>Sources thermales (composition chimique)</term><term>Sources thermales (microbiologie)</term><term>Techniques de culture (MeSH)</term><term>Tunisie (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Hydrogensulfite Reductase</term><term>Oxidoreductases</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Archaea</term><term>Hot Springs</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Archéobactéries</term><term>Proteobacteria</term><term>Sources thermales</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Archaea</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Archéobactéries</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Sources thermales</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hot Springs</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Archéobactéries</term><term>Hydrogensulfite reductase</term><term>Interactions microbiennes</term><term>Oxidoreductases</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Archaea</term><term>Microbial Interactions</term><term>Proteobacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biota</term><term>Culture Techniques</term><term>Cytogenetic Analysis</term><term>Genes, rRNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse cytogénétique</term><term>Animaux</term><term>Biote</term><term>Gènes d'ARN ribosomique</term><term>Techniques de culture</term><term>Tunisie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Prokaryotic diversities of 12 geothermal hot springs located in Northern, Central and Southern Tunisia were investigated by culture-based and molecular approaches. Enrichment cultures for both aerobic and anaerobic microorganisms were successfully obtained at temperatures ranging from 50 to 75°C. Fourteen strains including four novel species were cultivated and assigned to the phyla Firmicutes (9), Thermotogae (2), Betaproteobacteria (1), Synergistetes (1) and Bacteroidetes (1). Archaeal or universal oligonucleotide primer sets were used to generate 16S rRNA gene libraries. Representative groups included Proteobacteria, Firmicutes, Deinococcus-Thermus, Thermotogae, Synergistetes, Bacteroidetes, Aquificae, Chloroflexi, candidate division OP9 in addition to other yet unclassified strains. The archaeal library showed a low diversity of clone sequences belonging to the phyla Euryarchaeota and Crenarchaeota. Furthermore, we confirmed the occurrence of sulfate reducers and methanogens by amplification and sequencing of dissimilatory sulfite reductase (dsrAB) and methyl coenzyme M reductase α-subunit (mcrA) genes. Altogether, we discuss the diverse prokaryotic communities arising from the 12 geothermal hot springs studied and relate these findings to the physico-chemical features of the hot springs.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Plouzané</li></settlement></list><tree><noCountry><name sortKey="Alain, Karine" sort="Alain, Karine" uniqKey="Alain K" first="Karine" last="Alain">Karine Alain</name><name sortKey="Barbier, Georges" sort="Barbier, Georges" uniqKey="Barbier G" first="Georges" last="Barbier">Georges Barbier</name><name sortKey="Birrien, Jean Louis" sort="Birrien, Jean Louis" uniqKey="Birrien J" first="Jean Louis" last="Birrien">Jean Louis Birrien</name><name sortKey="Hamdi, Mokhtar" sort="Hamdi, Mokhtar" uniqKey="Hamdi M" first="Mokhtar" last="Hamdi">Mokhtar Hamdi</name><name sortKey="Prieur, Daniel" sort="Prieur, Daniel" uniqKey="Prieur D" first="Daniel" last="Prieur">Daniel Prieur</name></noCountry><country name="France"><region name="Région Bretagne"><name sortKey="Sayeh, Raja" sort="Sayeh, Raja" uniqKey="Sayeh R" first="Raja" last="Sayeh">Raja Sayeh</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000113 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000113 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20835839
|texte= Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20835839" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |